专利详情

专利名称	Crystal structure of amino terminal portion of influenza virus polymerase PA subunit and use thereof
申请号	US201013254823	专利类型	US
公开（公告）号	US9017960(B2)	公开（授权）日	2015.04.28
申请（专利权）人	中国科学院生物物理研究所	发明（设计）人	Liu Yingfang;Rao Zihe
主分类号	C12Q1/34	IPC主分类号	C12Q1/34;C07K14/005;C12N9/12;C40B30/02;G06F19/16;G06F19/00
专利有效期	Crystal structure of amino terminal portion of influenza virus polymerase PA subunit and use thereof 至Crystal structure of amino terminal portion of influenza virus polymerase PA subunit and use thereof	法律状态
说明书摘要	Present invention discloses the three-dimensional crystal structure of the N-terminus polypeptide of influenza virus polymerase subunit (PA_N of SEQ ID NO:7). PA_N of SEQ ID NO: 1 is residues 1—50 to 150-300 of influenza virus polymerase subunit PA. In the three-dimensional structure, at least 40% of atoms show the same atomic coordinates, compared to that listed in Table 1. Namely, in the three-dimensional structure of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, 40% of atomic coordinates on carbon skeleton of residues of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, show less than or equal to 1.7 ? of average variance, compared to the atomic coordinates listed in Table 1. Present invention also discloses the expression, purification, crystallization methods, and three-dimensional crystal structure of 256 residues in the N-terminus of influenza virus polymerase subunit PA, and applications of the crystal structure of SEQ ID NO: 7 on drug screening and designing.