专利详情

专利名称	Recombinant flagellin protein and preparation and use thereof
申请号	US201013122409	专利类型	US
公开（公告）号	US8449891(B2)	公开（授权）日	2013.05.28
申请（专利权）人	中国科学院武汉病毒研究所	发明（设计）人	YAN HUIMINLIU FANGYANG JINGY
主分类号	C07K1/00	IPC主分类号	C07K1/00;A61K39/00;A61K39/02;A61K39/112;A61K39/38;C07K14/00;C07K17/00
专利有效期	Recombinant flagellin protein and preparation and use thereof 至Recombinant flagellin protein and preparation and use thereof	法律状态
说明书摘要	The present invention provides an optimized recombinant flagellin protein and preparation and use thereof. The protein is with a deletion in the hypervariable region, said hypervariable region is the region from 180 to 400 amino acid of the flagellin protein, and the proteins include FliCDelta190-278, FliCDelta220-320 or FliCDelta180-400. The method of preparing said protein, comprising introducing a deletion into the hypervariable region of the flagellin protein. First constructed the flagellin protein recombinant plasmid, and then used it as template to construct the flagellin deletion cloning, and expressed and purified. The present invention also provides the use of the recombinant flagellin protein as adjuvant. The recombinant flagellin protein in present invention decreases the potential risks it may have, and decreases its antigenicity and immunogenicity and the inflammatory response induced by it, through deleting its main areas of immunogenicity and antigen activity.