专利详情

专利名称	METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL
申请号	EP20070721024	专利类型	EP
公开（公告）号	EP2023138(B1)	公开（授权）日	2012.03.21
申请（专利权）人	中国科学院上海生命科学研究院	发明（设计）人	ZHOU ZHUANCHENG HEPIN
主分类号	G01N33/50	IPC主分类号	G01N33/50
专利有效期	METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL 至METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL	法律状态
说明书摘要	The invention relates to methods and system for detecting ligand binding to membrane receptors and endocytosis. A method for detecting ligand binding to a membrane receptor includes the steps of: incubating the cell with a dye and a ligand; and detecting dye-containing endocytic vesicles in the cell. A system for screening ligand binding to membrane receptors includes an automatic liquid handling device; an x-y stage for positioning a plate; a microscope configured to image endocytic vesicles of a cell; a software for automatic analysis of FM spots (endocytic vesicles); and a control unit configured to control the movement of the x-y stage and the microscope.