专利详情

专利名称	Gutless adenovirus vector and the construction method thereof
申请号	US20040559008	专利类型	US
公开（公告）号	US2007077226	公开（授权）日	2007.04.05
申请（专利权）人	中国科学院上海生命科学研究院	发明（设计）人	LIU XINYUAN (CN);PEI ZIFEL (CN);LI BINGHUA (CN);GU JINFA (CN);ZOU WEIGUO (CN);SUN LANYING (CN)
主分类号	A61K48/00	IPC主分类号	A61K48/00;A61P35/00;C12N7/01;C12N15/861;A61K38/00
专利有效期	Gutless adenovirus vector and the construction method thereof 至Gutless adenovirus vector and the construction method thereof	法律状态
说明书摘要	The present invention disclosed a kind of gutless adenovirus vector and the construction method thereof. Two structural independent but functional related cassettes, the trans-activator (TA) and anti-tumor cassette, are both carried by the gutless vector. hTERT promoter restricts the expression of TA only in tumor cells, and RU486, associated with TA, regulates the expression of interesting gene: when needed, add the RU486 and the gene expression is on, and when not needed, remove the RU486 and the gene expression is off. Tumor-specificity and small molecule regulation of the vector spare the toxicity to the normal tissue caused by the foreign gene product and endow the gene's long lifetime expression in vivo. The vector of the present invention shows many advantages over traditional adenovirus vectors in targeting, gene regulation and expression lifetime.