专利详情

专利名称	Recombinant vaccinia virus
申请号	US26959388A	专利类型	US
公开（公告）号	US5077213A	公开（授权）日	1991.12.31
申请（专利权）人	中国科学院上海生物化学研究所	发明（设计）人	LI ZAI-PING ; WANG YUAN
主分类号	C12N7/01	IPC主分类号	C12N7/01
专利有效期	Recombinant vaccinia virus 至Recombinant vaccinia virus	法律状态
说明书摘要	A recombinant vaccinia virus, effective in combatting hepatitis B infections in humans, is provided. The virus has incorporated therein the entire HBsAg gene, and is capable of expressing in and secreting from animal (including human) cells, in particulate form, all three of the constituent protein epitopes S, preS2 and preS1 constituting the proteinaceous surface antigen of hepatitis B virus. The recombinant vaccinia virus is prepared by recombination of a selected vaccinia virus strain of very low toxicity towards mammals with an expression plasmid containing the HBsAg sequence and the promoter sequence thereof in operative registry therewith, this sequence being provided at a location interposed within a gene sequence common to the expression plasmid and the vaccinia viral genome. This recombinant virus can be used as a novel live vaccine of low toxicity and also as a system for the production of secretable HBsAg particles with all of the three components, which can be applied as a novel sub-unit vaccine.