专利详情

专利名称	DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
申请号	US19980157397	专利类型	US
公开（公告）号	US6165765	公开（授权）日	2000.12.26
申请（专利权）人	中国科学院上海生物化学研究所	发明（设计）人	HONG GUOFAN (CN); HUANG WEI-HUA (CN)
主分类号	C12N9/12	IPC主分类号	C12N9/12;C12Q1/68;C12P19/34
专利有效期	DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides 至DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides	法律状态
说明书摘要	The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.