Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity
- 申请号:US64268496A
- 专利类型:US
- 申请(专利权)人:中国科学院上海生物化学研究所
- 公开(公开)号:US5834253A
- 公开(公开)日:1998.11.10
- 法律状态:
- 出售价格: 面议 立即咨询
专利详情
专利名称 | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity | ||
申请号 | US64268496A | 专利类型 | US |
公开(公告)号 | US5834253A | 公开(授权)日 | 1998.11.10 |
申请(专利权)人 | 中国科学院上海生物化学研究所 | 发明(设计)人 | HONG GUO FAN ; HUANG WEI-HUA ; ZHAI DECEASED FENG |
主分类号 | C12N9/12 | IPC主分类号 | C12N9/12;C12N15/54;C12P19/34 |
专利有效期 | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity 至Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity | 法律状态 | |
说明书摘要 | The invention relates to DNA polymerases which are capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to isolated and cloned DNA sequences derived from the Bacillus stearothermophilus thermostable DNA polymerase, as well as the expressed polymerase itself. |
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