专利详情

专利名称	DNA polymerase with proof-reading 3'-5' exonuclease activity Bacillus stearothermophilus
申请号	US19950544643	专利类型	US
公开（公告）号	US5747298	公开（授权）日	1998.05.05
申请（专利权）人	中国科学院上海生物化学与细胞生物学研究所	发明（设计）人	HONG GUO FAN (CN); ZHAI DECEASED FENG (CN)
主分类号	C12P19/34	IPC主分类号	C12P19/34;C12N9/12;C12N1/20
专利有效期	DNA polymerase with proof-reading 3'-5' exonuclease activity Bacillus stearothermophilus 至DNA polymerase with proof-reading 3'-5' exonuclease activity Bacillus stearothermophilus	法律状态
说明书摘要	The invention relates to a thermostable Bacillus stearothermophilus DNA polymerase which is capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to strains of Bacillus stearothermophilus capable of producing the thermostable DNA polymerase.