专利详情

专利名称	Triplex forming oligonucleotides and their use in anti-HBV
申请号	US20000529812	专利类型	US
公开（公告）号	US6682930(B1)	公开（授权）日	2004.01.27
申请（专利权）人	中国科学院上海生物化学与细胞生物学研究所	发明（设计）人	LU CHANGDE (CN)
主分类号	C07H21/04	IPC主分类号	C07H21/04;C12N15/09;A61P31/00;A61K38/0020060101C;C12N15/86;C12N15/85;C12Q1/68;C07H21/0020060101C;C07H21/0020060101A;C12P19/34;A61P31/20;C12N15/11320100101C;A61K48/00;C12N15/11320100101A;A61K31/711;A61K38/0020060101A
专利有效期	Triplex forming oligonucleotides and their use in anti-HBV 至Triplex forming oligonucleotides and their use in anti-HBV	法律状态
说明书摘要	A type of new triplex forming oligonucleotide (TFO) which can form triplex DNA when bound to two similar fragments of homopolypurine/homopolypyrimidine sequences. TFOs were designed according to the above structure to bind the DR region and pre-S gene promoter region of HBV, respectively. The 3'' end of the TFOs can be monophosphorylated or otherwise chemically modified to increase their stability. Cellular experiments prove that these TFOs can be used as drugs to inhibit HBV and treat hepatitis B. TFO and anti-sense oligonucleotide sequences from the DR or pre-S promoter region of HBV can together bind to target RNA sequences and form a (DNA)2:RNA hetero-triplex structure that results in the more efficient inhibition of HBV.